|
Enzyme |
Source |
Sequence cut |
Average expected fragment size (kb) in human DNAa |
|
Alu I |
Arthrobacter luteus |
AGCT |
0.3 |
|
Hae III |
Hemophilus aegyptus |
GGCC |
0.6 |
|
Taq I |
Thermus aquaticus |
TCGA |
1.4 |
|
Mnl I |
Moraxella nonliquefaciens |
CCTC/GAGG |
0.4 |
|
Hind III |
Hemophilus influenzae Rd |
AAGCTT |
3.1 |
|
EcoR I |
Escherichia Coli R factor |
GAATTC |
3.1 |
|
BamH I |
Bacillus amyloliquefaciens |
GGATCC |
7.0 |
|
Pst I |
Providencia stuartii |
CTGCAG |
7.0 |
|
Mst I |
Microcoleus species |
CCTNAGGc |
7.0 |
|
Sma I |
Serratia marcescens |
CCCGGG |
78 |
|
BssH II |
Bacillus stearothermophilus |
GCGCGC |
390b |
|
Not I |
Norcadia otitidis-caviarum |
GCGGCCGC |
9766 b |
|
(a) Assuming 40% G+C, and a CpG frequency 20% of that expected (b) Observed average sizes are considerably lower than these estimates © N = A, C, G or T Notes: Names are normally derived from the first letter of the genus and the first two letters of the species name, e.g. Pst I is the first restriction nuclease to have been isolated from Providencia stuartii. Mnl I is an example of an enzyme whose recognition sequence is not palindromic. So-called "rare-cutters" often have recognition sequences containing one or more CpG dinucleotides and cut vertebrate DNA comparatively infrequently. |
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