Title Page

Objective

 General Introduction

Genetic Markers

Definition
Phenotypic Markers
 Blood Groups
Biochemical Polymorphisms

RFLPs

 RFLPs = Restriction Fragment Length Polymorphisms = variation in the restriction map of a given locus

 Restrcition enzymes:

Restriction endonucleases cut DNA molecules at specific sites (1 )

Recognition sequences for the majority of Type II restriction endonucleases are palindromes, usually 4-8 bp long (1 )

 RFLPs can result from:

Point mutation creating or destroying a restriction site (1 )

Insertion / deletions altering the length of a given restriction fragment.

 RFLPs are usually detected by Southern blotting (1 )

 A seminal paper based on RFLPs has been the start point of a new era in mammalian genetics (Botstein et al., 1980)

 Minisatellites or VNTR Markers
 Microsatellites or SSR Markers
 SNPs

 Map Construction

QTL Mapping in Outbred Half-Sib Pedigrees

References