In 1980, Wyman and White describe the first polymorphism due to allelic variation in the number of tandem repeats of a minisatellite (1
). They call these sequences VNTRs (Variable Number of Tandem Repeats).
VNTR markers are usually genotyped using Southern blotting using restriction enzymes cutting in the sequences flanking the VNTR (1
)
In 1985, Jeffreys demonstrates that minisatellites are organized in families of related sequences and uses this property to develop DNA fingerprinting systems (1 1 )
VNTR markers and DNA fingerprints have been used extensively for linkage analysis because of their high informativeness (heterozygosities > 70% are common) but suffer from their uneven genomic distribution.
Title Page
General
IntroductionDefinition
Minisatellites or VNTR Markers