Dynamic
allele-specific hybridization W. Mathias Howell et
al.
Nature Biotechnology 17, 87 - 88
(1999)
Figure 2: DASH model experiment.
DASH was performed on two allelic oligonucleotide targets of
sequences biotin: 5´-ATTAGGG(T/C)GGGGAGA-3´ (alternatives shown in
parentheses). The assay probe was 5´-TCTCCCC(G)CCCTAAT-3´. (A) The
resulting fluorescence changes against temperature. (B) The negative
first derivatives. The oligonucleotide matching the probe (), the single-base
mismatched oligonucleotide (),
and plain lines representing a 50/50 oligonucleotide
mixture.
), the single-base
mismatched oligonucleotide (.gif)
),
and plain lines representing a 50/50 oligonucleotide
mixture.